Wheat germ cell-free platform for eukaryotic protein production
نویسندگان
چکیده
منابع مشابه
Wheat germ cell-free system-based production of malaria proteins for discovery of novel vaccine candidates.
One of the major bottlenecks in malaria research has been the difficulty in recombinant protein expression. Here, we report the application of the wheat germ cell-free system for the successful production of malaria proteins. For proof of principle, the Pfs25, PfCSP, and PfAMA1 proteins were chosen. These genes contain very high A/T sequences and are also difficult to express as recombinant pro...
متن کاملTranslation of albumin messenger RNA in a cell-free protein-synthesizing system derived from wheat germ.
Purified rat liver albumin mRNA directed the synthesis of albumin in a mRNA-dependent cell-free protein-synthesizing system derived from wheat germ extracts. The [3H]leucine-labeled in vitro translation product reacted with antibodies specific for albumin and co-migrated with authentic 14C-labeled serum albumin during gel electrophoresis in the presence or absence of sodium dodecyl sufate. High...
متن کاملAn integrated cell-free metabolic platform for protein production and synthetic biology
Cell-free systems offer a unique platform for expanding the capabilities of natural biological systems for useful purposes, i.e. synthetic biology. They reduce complexity, remove structural barriers, and do not require the maintenance of cell viability. Cell-free systems, however, have been limited by their inability to co-activate multiple biochemical networks in a single integrated platform. ...
متن کاملWheat germ cell-free expression system as a pathway to improve protein yield and solubility for the SSGCID pipeline
Recombinant expression of proteins of interest in Escherichia coli is an important tool in the determination of protein structure. However, lack of expression and insolubility remain significant challenges to the expression and crystallization of these proteins. The SSGCID program uses a wheat germ cell-free expression system as a rescue pathway for proteins that are either not expressed or ins...
متن کاملIn vitro synthesis of turnip yellow mosaic virus coat protein in a wheat germ cell-free system.
Turnip Yellow Mosaic Virus RNA directs the synthesis in vitro of its coat protein in a wheat germ cell-free extract. Optimum conditions for synthesis have been defined, and the effect of spermine on specifically enhancing coat protein formation has been examined. Identity between the in vitro synthesized coat protein and authentic coat protein of Turnip Yellow Mosaic Virus was established by an...
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ژورنال
عنوان ژورنال: FEBS Journal
سال: 2006
ISSN: 1742-464X,1742-4658
DOI: 10.1111/j.1742-4658.2006.05434.x